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Proceedings Paper

Use of diode laser energy (808 nm) for selective photothermolysis of contaminated wounds
Author(s): Kenneth Eugene Bartels D.V.M.; Rebecca J. Morton; D. Thomas Dickey; Ernest L. Stair D.V.M.; Marie E. Payne; Steven A. Schafer; Robert E. Nordquist
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Paper Abstract

The objective of this study was to compare the antibacterial effects of selective laser treatments on contaminated wounds. Comparisons were made using the diode (808 nm) laser with and without an accompanying chromophore, Nd:YAG (1064 nm), and KTP (532 nm) lasers. A known quantity (mean concentration of 7.9 +/- 1.9 CFU/ml X 108) of bacterial organisms (Staphylococcus intermedius) was inoculated into skin incisions aseptically made on the backs of experimental rabbits. The bacteria were allowed to adhere/colonize for one hour, and each site was then treated with various laser modalities. Tissue samples were taken at one and forty-eight hours after treatment. The number of viable bacteria was then quantitated (CFU/ml) by a spot-plate counting technique. Initial results illustrated the fact that selective photothermolysis of superficial cellular layers of targeted tissue occurred with an accompanying decrease of bacterial numbers that virtually created a `clean' (organisms numbers less than 105 per gram of tissue) wound. This effect was especially noticeable using the diode laser (808 nm) and an accompanying chromophore, indocyanine green.

Paper Details

Date Published: 12 May 1995
PDF: 5 pages
Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); doi: 10.1117/12.209149
Show Author Affiliations
Kenneth Eugene Bartels D.V.M., Oklahoma State Univ. College of Veterinary Medicine (United States)
Rebecca J. Morton, Oklahoma State Univ. College of Veterinary Medicine (United States)
D. Thomas Dickey, Oklahoma State Univ. College of Veterinary Medicine (United States)
Ernest L. Stair D.V.M., Oklahoma State Univ. College of Veterinary Medicine (United States)
Marie E. Payne, Oklahoma State Univ. College of Veterinary Medicine (United States)
Steven A. Schafer, Oklahoma State Univ. (United States)
Robert E. Nordquist, Dean A. McGee Eye Institute (United States)


Published in SPIE Proceedings Vol. 2395:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V
R. Rox Anderson M.D.; Graham M. Watson M.D.; Rudolf W. Steiner; Douglas E. Johnson M.D.; Stanley M. Shapshay M.D.; Michail M. Pankratov; George S. Abela M.D.; Lawrence S. Bass M.D.; John V. White M.D.; Rodney A. White M.D.; Kenneth Eugene Bartels D.V.M.; Lloyd P. Tate V.D.M.; C. Thomas Vangsness M.D., Editor(s)

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