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Proceedings Paper

Oxidatively modified low-density lipoprotein in mononuclear cells detected by laser-induced fluorescence spectroscopy
Author(s): Tami N. Glenn; Alexander A. Oraevsky; Frank K. Tittel; Sharon L. Thomsen; Steven L. Jacques; Philip D. Henry
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Paper Abstract

Hyperlipidemic states are associated with focal accumulations in arterial walls of oxidatively modified low density lipoprotein (LDL) and monocyte-derived liquid-laden macrophages, biochemical and cellular hallmarks of atheromatous lesions. Mechanisms underlying the generation and cellular uptake of oxidized LDL are still incompletely understood. We have used laser-induced fluorescence spectroscopy to monitor the formation, intracellular accumulation, and tissue distribution of oxidized LDL. Oxidatively modified LDL excited by a XeCl excimer laser (308 nm) exhibits unique spectral characteristics that distinguish it from native (non-oxidized) LDL. The same spectral characteristics were demonstrated in lipid-rich atheromatous lesions, macrophages after incubation with oxidized LDL, and peripheral blood monocytes from hyperlipidemic, but not normolipidemic subjects. Detection of oxidized LDL in peripheral blood monocytes and arterial tissue may provide information on the atherogenic activity of hyperlipidemic states and serve as a novel risk factor for the assessment of atherosclerosis.

Paper Details

Date Published: 12 May 1995
PDF: 8 pages
Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); doi: 10.1117/12.209122
Show Author Affiliations
Tami N. Glenn, Rice Univ. (United States)
Alexander A. Oraevsky, Rice Univ. (United States)
Frank K. Tittel, Rice Univ. (United States)
Sharon L. Thomsen, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Steven L. Jacques, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Philip D. Henry, Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 2395:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V
R. Rox Anderson; Graham M. Watson; Rudolf W. Steiner; Douglas E. Johnson; Stanley M. Shapshay; Michail M. Pankratov; George S. Abela; Lawrence S. Bass; John V. White; Rodney A. White; Kenneth Eugene Bartels; Lloyd P. Tate; C. Thomas Vangsness, Editor(s)

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