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Proceedings Paper

Diagnostic fluorescence spectroscopy of oral mucosa
Author(s): Krishnendu Roy; Ian Bottrill; Duncan R. Ingrams; Michail M. Pankratov; Elie E. Rebeiz; Peak Woo; Sadru Kabani; Stanley M. Shapshay; Ramasamy Manoharan; Irving Itzkan; Michael S. Feld
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Paper Abstract

Autofluorescence characteristics of normal, dysplastic, and malignant squamous tissues from the oral cavity were measured with a spectrofluorometer in the excitation range of 250 - 500 nm and emission range of 350 - 750 nm. Fluorescence excitation-emission matrices (EEM) were obtained from samples collected from patients in the clinic and in the operating room. The same samples were submitted for histopathological examination following spectroscopic measurements. The contour plots obtained from the EEMs of the samples showed consistent differences between normal and abnormal tissues. All the abnormal samples showed enhanced red region (> 600 nm) fluorescence with a prominent peak at 635 nm, when excited by 410 nm light. A ratio contour plot (abnormal/normal) enhanced spectral differences in the red region. A fiber-optic based spectrofluorometer for EEM measurements is being developed for further investigations.

Paper Details

Date Published: 12 May 1995
PDF: 8 pages
Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); doi: 10.1117/12.209094
Show Author Affiliations
Krishnendu Roy, New England Medical Ctr./Tufts Univ. School of Medicine and Boston Univ. (United States)
Ian Bottrill, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Duncan R. Ingrams, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Michail M. Pankratov, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Elie E. Rebeiz, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Peak Woo, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Sadru Kabani, Tufts Univ. School of Dental Medicine (United States)
Stanley M. Shapshay, New England Medical Ctr./Tufts Univ. School of Medicine (United States)
Ramasamy Manoharan, Massachusetts Institute of Technology (United States)
Irving Itzkan, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 2395:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V
R. Rox Anderson; Graham M. Watson; Rudolf W. Steiner; Douglas E. Johnson; Stanley M. Shapshay; Michail M. Pankratov; George S. Abela; Lawrence S. Bass; John V. White; Rodney A. White; Kenneth Eugene Bartels; Lloyd P. Tate; C. Thomas Vangsness, Editor(s)

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