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Proceedings Paper

Perspectives in nanostructure assisted laser manipulation of mammalian cells
Author(s): Dag Heinemann; Markus Schomaker; Stefan Kalies; Anton Hoerdt; Hugo Murua Escobar; Tammo Ripken; Heiko Meyer
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Paper Abstract

The interaction of cell-adhered nanostructures with laser light has attracted much interest within the biomedical field. Molecular delivery using a variety of plasmonic nanostructures, such as structured surfaces, nanoparticles and particle clusters, is currently evolving from its proof-of-concept into a routine method. Here, gold represents the material of choice, as it provides unique optical properties, different surface modifications as well as biocompatibility. In addition, new materials (e.g. polypyrrole) provide interesting alternatives.

Applying this approach, a variety of molecules, such as fluorescent dyes, proteins, antisense structures, and DNA, has been transfected in order to manipulate the cellular functions in different experimental settings. Antisense structures, for example, allow the efficient down regulation of the gene activity of a target, providing insights into the gene’s function. The delivery of proteins, as executing molecules in the cell, can exhibit an immediate effect on the cell behavior, allowing a minute observation of the intracellular kinetics.

Direct cell manipulation can be achieved with this approach as well. Increasing the nanoparticle concentration and/or the radiant exposure, effective cell destruction is induced. Using targeted nanoparticles (e.g. by antibody conjugation) in combination with spatially selective laser irradiation permits well-directed cell manipulation even in mixed cultures and potentially in tissues. Furthermore, excited gold nanoparticles can directly trigger cellular reactions, which can possibly be utilized for cell stimulation. The manifold possibilities of nanostructure assisted laser manipulation are still in development.

Paper Details

Date Published: 9 March 2015
PDF: 6 pages
Proc. SPIE 9355, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV, 935509 (9 March 2015); doi: 10.1117/12.2086679
Show Author Affiliations
Dag Heinemann, Laser Zentrum Hannover e.V. (Germany)
Markus Schomaker, Laser Zentrum Hannover e.V. (Germany)
Stefan Kalies, Laser Zentrum Hannover e.V. (Germany)
Anton Hoerdt, Laser Zentrum Hannover e.V. (Germany)
Hugo Murua Escobar, Univ. of Rostock (Germany)
Tammo Ripken, Laser Zentrum Hannover e.V. (Germany)
Heiko Meyer, Laser Zentrum Hannover e.V. (Germany)


Published in SPIE Proceedings Vol. 9355:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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