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Proceedings Paper

Quantitative detection of bovine and porcine gelatin difference using surface plasmon resonance based biosensor
Author(s): Devy Pramudyah Wardani; Muhammad Arifin; Edi Suharyadi; Kamsul Abraha
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Paper Abstract

Gelatin is a biopolymer derived from collagen that is widely used in food and pharmaceutical products. Due to some religion restrictions and health issues regarding the gelatin consumption which is extracted from certain species, it is necessary to establish a robust, reliable, sensitive and simple quantitative method to detect gelatin from different parent collagen species. To the best of our knowledge, there has not been a gelatin differentiation method based on optical sensor that could detect gelatin from different species quantitatively. Surface plasmon resonance (SPR) based biosensor is known to be a sensitive, simple and label free optical method for detecting biomaterials that is able to do quantitative detection. Therefore, we have utilized SPR-based biosensor to detect the differentiation between bovine and porcine gelatin in various concentration, from 0% to 10% (w/w). Here, we report the ability of SPR-based biosensor to detect difference between both gelatins, its sensitivity toward the gelatin concentration change, its reliability and limit of detection (LOD) and limit of quantification (LOQ) of the sensor. The sensor’s LOD and LOQ towards bovine gelatin concentration are 0.38% and 1.26% (w/w), while towards porcine gelatin concentration are 0.66% and 2.20% (w/w), respectively. The results show that SPR-based biosensor is a promising tool for detecting gelatin from different raw materials quantitatively.

Paper Details

Date Published: 5 May 2015
PDF: 8 pages
Proc. SPIE 9506, Optical Sensors 2015, 95060W (5 May 2015); doi: 10.1117/12.2086259
Show Author Affiliations
Devy Pramudyah Wardani, Univ. Gadjah Mada (Indonesia)
Muhammad Arifin, Univ. Gadjah Mada (Indonesia)
Edi Suharyadi, Univ. Gadjah Mada (Indonesia)
Kamsul Abraha, Univ. Gadjah Mada (Indonesia)

Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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