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Proceedings Paper

In-situ histochemical analysis of human coronary artery by Raman spectroscopy compared with biochemical assay
Author(s): James F. Brennan; Tjeerd J. Roemer; Anna M. Tercyak; Yang Wang; Maryann Fitzmaurice; Robert S. Lees; John R. Kramer; Ramachandra R. Dasari; Michael S. Feld
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Paper Abstract

We have developed a method to analyze quantitatively the biochemical composition of human coronary artery in situ using near infrared Raman spectroscopy. Human coronary arteries were obtained from explanted hearts after heart transplantation. Samples of normal intima/media, adventitia, non-calcified and calcified plaque were illuminated with 830 nm light from a CW Ti:Sapphire laser. The Raman scattered light was collected and coupled into a 1/4 meter spectrometer that dispersed the light onto a liquid nitrogen cooled, deep-depletion CCD detector. Raman spectra with sufficiently high S/N for extracting biochemical information could be collected in under one second. The spectra were analyzed using a recently developed model to quantitate the relative weight fractions of cholesterol, cholesterol esters, triacylglycerol, phospholipids, protein, and calcium salts. After spectral examination, the artery samples were biochemically assayed to determine the total lipid weight and the amount of the major lipid categories as a percentage of the total lipid content. The results of the lipid biochemical assay and the Raman spectral model compare favorably, indicating that relative lipid weights can be accurately determined in situ. Protein and calcium salts assays are underway. This in situ biochemical information may be useful in diagnosing atherosclerosis and studying disease progression.

Paper Details

Date Published: 8 May 1995
PDF: 5 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208528
Show Author Affiliations
James F. Brennan, Massachusetts Institute of Technology (United States)
Tjeerd J. Roemer, Cleveland Clinic Foundation (United States)
Anna M. Tercyak, Boston Univ. School of Medicine (United States)
Yang Wang, Massachusetts Institute of Technology (United States)
Maryann Fitzmaurice, Case Western Reserve Univ. (United States)
Robert S. Lees, Harvard Univ. and Massachusetts Institute of Technology (United States)
John R. Kramer, Cleveland Clinic Foundation (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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