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Proceedings Paper

Two-dimensional infrared fluorescence scanner used for DNA analysis
Author(s): Lyle Richard Middendorf; Robert C. Bruce; John A. Brumbaugh; Daniel L. Grone; Gi Y. Jang; Peter Richterich; Holges Joachim Hoeltke; Richard J. Williams; Mauro Dr. Peralta
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Paper Abstract

A LI-COR Model 4000 DNA Sequencer has been modified by removing the internal scanning infrared fluorescence microscope and combining it with an external, orthogonal scanner. Due to the reduced background fluorescence and light scattering of nylon membranes in the near- infrared (8000 nm) as compared to the visible region of the optical spectrum, sensitivity of labeled DNA fragments is enhanced. Dot blots of dilution series of labeled oligonucleotides reveal a detection limit of 25 attomole (25 X 10-18 mole). DNA fragments blotted onto nylon membranes using direct transfer electrophoresis in multiplex DNA sequencing can also be detected and subsequently analyzed.

Paper Details

Date Published: 8 May 1995
PDF: 12 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208522
Show Author Affiliations
Lyle Richard Middendorf, LI-COR, Inc. (United States)
Robert C. Bruce, LI-COR, Inc. (United States)
John A. Brumbaugh, LI-COR, Inc. (United States)
Daniel L. Grone, LI-COR, Inc. (United States)
Gi Y. Jang, LI-COR, Inc. (United States)
Peter Richterich, Genome Therapeutics Corp. (United States)
Holges Joachim Hoeltke, Boehringer Mannheim GmbH (Germany)
Richard J. Williams, Georgia State Univ. (United States)
Mauro Dr. Peralta, Univ. Federal do Rio de Janeiro (Brazil)
Centers for Disease Control/Atlanta (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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