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Proceedings Paper

Integrated optic immunoassay for virus detection
Author(s): Anthony A. Boiarski; James R. Busch; Larry S. Miller; A. W. Zulich; James Burans
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Paper Abstract

An integrated optic refractometer device was developed to perform a rapid one-step, label-free immunoassay. The device measures refractive index changes at the surface of a planar waveguide using interferometry. Antibodies were applied to the waveguide surface to provide a bioselective coating for detecting and quantifying a specific antigen of interest. The detection limit of this biosensor was determined for adenovirus as a model for other viral analytes of military, medical, and environmental interest. As binding of the antigen occurred on the sensor surface, a time-dependent phase shift of the helium-neon laser light beam was detected and was measured over a 10-minute time period. Adenovirus was detected at levels of 250 - 2500 viral particles/ml. This detection limit was obtained for a mono-layer of antibody attached to the sensor. Use of a high-density, multi-layer antibody coating approach resulted in improved detection limits for bacteria and protein analytes of general interest.

Paper Details

Date Published: 8 May 1995
PDF: 9 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208519
Show Author Affiliations
Anthony A. Boiarski, Battelle Memorial Institute/Columbus Div. (United States)
James R. Busch, Battelle Memorial Institute/Columbus Div. (United States)
Larry S. Miller, Battelle Memorial Institute/Columbus Div. (United States)
A. W. Zulich, Edgewood Research, Development and Engineering Ctr. (United States)
James Burans, Naval Medical Research Institute (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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