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Proceedings Paper

Development of a penicillin biosensor using a single optical imaging fiber
Author(s): Brian G. Healey; David R. Walt
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Paper Abstract

A penicillin biosensor has been fabricated by photodepositing penicillin-sensitive polymer matrices and pH-sensitive polymer matrices on different regions of an optical imaging fiber. Penicillin is detected by coupling the enzymatic activity of penicillinase with the pH sensitivity of fluorescein. Penicillin concentration is correlated to the pH change in the microenvironment of the penicillin-sensitive matrix relative to the pH of the sample solution. This dual sensor removes the need to maintain a constant solution pH when measuring penicillin and should enhance greatly the application of biosensors.

Paper Details

Date Published: 8 May 1995
PDF: 6 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208518
Show Author Affiliations
Brian G. Healey, Tufts Univ. (United States)
David R. Walt, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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