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Proceedings Paper

Blood characterization using UV/vis spectroscopy
Author(s): Yvette D. Mattley; F. Mitrani-Gold; S. Orton; Christina P. Bacon; German F. Leparc; M. Bayona; Robert L. Potter; Luis H. Garcia-Rubio
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Paper Abstract

The current methods used for typing blood involve an agglutination reaction which results from the association of specific antibodies with antigens present on the erythrocyte cell surface. While this method is effective, it requires involved laboratory procedures to detect the cell surface antigens. As an alternative technique, uv/vis spectroscopy has been investigated as a novel way to characterize and differentiate the blood types. Typing with this technique is based on spectral differences which appear throughout portions of both the ultraviolet and visible range. The origin of these spectral differences is unknown and presently under investigation. They may be due to intrinsic absorption differences at the molecular level, and/or they may be due to scattering differences brought about by either subtle variation in cell surface characteristics, cell shape or state of aggregation. As the background optical density in these samples is identified and accounted for, the spectral differences become more defined. This work and the continuation of this project will be included in a general database encompassing a wide range of blood samples. In addition, long term goals involve the investigation of diseased blood with the potential of providing a more rapid diagnosis for blood borne pathogens.

Paper Details

Date Published: 8 May 1995
PDF: 9 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208508
Show Author Affiliations
Yvette D. Mattley, Univ. of South Florida (United States)
F. Mitrani-Gold, Univ. of South Florida (United States)
S. Orton, Florida Blood Services (United States)
Christina P. Bacon, Univ. of South Florida (United States)
German F. Leparc, Florida Blood Services (United States)
M. Bayona, Univ. of South Florida (United States)
Robert L. Potter, Univ. of South Florida (United States)
Luis H. Garcia-Rubio, Univ. of South Florida (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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