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Proceedings Paper

Fiber optic monitoring system for mouse embryo evaluation
Author(s): Harumi Itoh; Tsunenori Arai; Makoto Kikuchi
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Paper Abstract

We constructed a fiber-optic long-term monitoring system for mouse embryo, which developed a new quantitative and non-invasive evaluation method for human embryo. We monitored the changes of light scattering from mouse embryo which might relate to the state of blastomere and change in intercellular microstructures. We employed a couple of optical fibers to measure the scattering. These fibers were arranged in a light angle to the optical axis of a microscope to obtain adaptability for various measurements. A He-Ne laser with 632.8 nm in wavelength was used as a light source. The emission fiber (56 micrometers (Phi) ; core) and sensing fiber (400 micrometers (Phi) ; core, NA equals 0.43) were used. We observed the light scattering changes from the embryo, which were over 200 times higher than the noise level with 30 s in period. This result might be attributed to the structural and/or functional changes in the mouse embryo.

Paper Details

Date Published: 8 May 1995
PDF: 4 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208507
Show Author Affiliations
Harumi Itoh, National Defense Medical College (Japan)
Tsunenori Arai, National Defense Medical College (Japan)
Makoto Kikuchi, National Defense Medical College (Japan)


Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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