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Proceedings Paper

Spectral inhomogeneity and intermolecular relaxation in erythrocyte ghosts and phospholipid bilayer membranes
Author(s): Nicolai A. Nemkovich; Alexander S. Kozlovski; Anatoly Nikolaevich Rubinov
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Paper Abstract

Using subnanosecond laser spectrofluorometry, the spectral and polarization time-resolved characteristics of 1-phenylnaphthylamine (1-AN) fluorescent probe in phospholipid bilayer and red blood cell membranes have been studied. It is shown that the electronic spectra of probe in model membranes are inhomogeneously broadened. In contrast to low-molecular weight solvents there are two reasons for inhomogeneous broadening. The first is connected with different levels of location of probe molecules in the membrane. Inhomogeneous broadening due to the first factor has a static character. The second reason is similar to that of solutions and linked with fluctuations of solvate structure. This type of broadening has a dynamic character. The process of intermolecular relaxation in membranes is accompanied by the release of the free energy excess, which results in wavelength-dependent rotation of probe.

Paper Details

Date Published: 8 May 1995
PDF: 11 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208498
Show Author Affiliations
Nicolai A. Nemkovich, B. I. Stepanov Institute of Physics (Belarus)
Alexander S. Kozlovski, B. I. Stepanov Institute of Physics (Belarus)
Anatoly Nikolaevich Rubinov, B. I. Stepanov Institute of Physics (Belarus)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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