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Proceedings Paper

Detection and investigation of photoinduced transient states of organic dyes by nonlinear probing method
Author(s): Olga V. Przhonska; Mikhail V. Bondar; Alexander P. Demchenko
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Paper Abstract

The absorption of high intensity laser light by dye molecular probes may produce non-linear effects. They result in both bleaching and darkening, which depends on the rates of depopulation/repopulation of the ground S0 and excited S1 states. It is shown, that the darkening is the characteristic features of fast photophysical and photochemical reactions occurring in the S1 state. This suggests a very simple method for detection and study of these reactions. The method is tested for photoinduced proton transfer and solute-solvent structural reorganizations in polymethine dyes. The results suggest, that the dye probes exhibiting these reactions may be applied for visualization of protonic gradients, microviscosity and distribution of different substances within the living cells. The method is based on the comparison of light transmission images at low and high intensities. It is extremely simple and fast, a single light pulse may be sufficient to create the image.

Paper Details

Date Published: 8 May 1995
PDF: 8 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208491
Show Author Affiliations
Olga V. Przhonska, Institute of Physics (Ukraine)
Mikhail V. Bondar, Institute of Physics (Ukraine)
Alexander P. Demchenko, A.V. Palladin Institute of Biochemistry (Ukraine)


Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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