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Proceedings Paper

Long-wavelength derivatization reagents for use in diode laser-induced fluorescence detection
Author(s): H. Thomas Karnes; Sadayappan V. Rahavendran; Min Gui
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Paper Abstract

A diode laser fluorometer was constructed and evaluated using rhodamine 800, a commercially available laser dye. Rhodamine 800 was evaluated as a precolumn derivatization reagent through an intermediate for conjugation to amine containing analytes. Preliminary results show that the derivative is nonfluorescent. Rhodamine 800 was successfully used as a mobile phase additive for HPLC with indirect detection of valproic acid from plasma. Nile blue, was evaluated as a derivatization reagent for carboxyl containing analytes. A limit of detection of 0.96 pmol was observed for the nile blue conjugate of benzoic acid using a conventional fluorescence detector. A cyanine dye, was used as a near infrared labeling reagent for compounds containing primary amine functionality. The spectral properties of the cyanine derivative of 2-adamantanamine were similar to the unreacted label and very favorable for low level detection.

Paper Details

Date Published: 8 May 1995
PDF: 11 pages
Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208489
Show Author Affiliations
H. Thomas Karnes, Medical College of Virginia (United States)
Sadayappan V. Rahavendran, Medical College of Virginia (United States)
Min Gui, Medical College of Virginia (United States)

Published in SPIE Proceedings Vol. 2388:
Advances in Fluorescence Sensing Technology II
Joseph R. Lakowicz, Editor(s)

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