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Proceedings Paper

A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy
Author(s): Dean Nicholas; Colin Fowley; Anthony P. McHale; Sukanta Kamila; Jason Sheng; Jordan Atchison; John F. Callan
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Paper Abstract

Folic acid (FA) has been used as a molecular targeting strategy to improve the specificity of a CQD-protoporphyrin IX (CQD-PPIX) conjugate to folate receptor positive (FR+) HeLa cells for use in two-photon excited Photodynamic Therapy (TPE-PDT). FA was covalently attached to the CQD-PPIX conjugate to form a FA-CQD-PPIX conjugate. The uptake of the FA-CQD-PPIX conjugate in FR+ HeLa cells was shown to be 7 times greater than the CQD-PPIX conjugate, while both conjugates showed a similar uptake in FR negative (FR-) HT-47 cells. TPE-PDT experiments, using HeLa cells as a target, revealed a 30% improved cytotoxicity for cells treated with the FA-CQD-PPIX conjugate and TPE compared to controls treated with the CQD-PPIX conjugate and TPE. Collectively, these results suggest the presence of FA can facilitate targeting of CQD-sensitiser conjugates to FR+ cells resulting in an improved PDT effect.

Paper Details

Date Published: 12 March 2015
PDF: 5 pages
Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 933813 (12 March 2015); doi: 10.1117/12.2084821
Show Author Affiliations
Dean Nicholas, Univ. of Ulster (United Kingdom)
Colin Fowley, Univ. of Ulster (United Kingdom)
Anthony P. McHale, Univ. of Ulster (United Kingdom)
Sukanta Kamila, Univ. of Ulster (United Kingdom)
Jason Sheng, Univ. of Ulster (United Kingdom)
Jordan Atchison, Univ. of Ulster (United Kingdom)
John F. Callan, Univ. of Ulster (United Kingdom)

Published in SPIE Proceedings Vol. 9338:
Colloidal Nanoparticles for Biomedical Applications X
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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