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Proceedings Paper

Fiber optic based fluorescence detection system for in vivo studies of exogenous chromophore pharmacokinetics
Author(s): Daniel R. Doiron; J. Brian Dunn; W. L. Mitchell; Brian K. Dalton; Greta M. Garbo; Jon A. Warner
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Paper Abstract

The detection and quantification of the concentration of exogenous chromophores in-vivo by their fluorescence is complicated by many physical and geometrical parameters. Measurement of such signals is advantageous in determining the pharmacokinetics of photosensitizers such as those used in photodynamic therapy (PDT) or to assist in the diagnosis of tissue histological state. To overcome these difficulties a ratio based fiber optic contact fluorometer has been developed. This fluorescence detection system (FDS) uses the ratio of the fluorescence emission peak of the exogenous chromophore to that of endogenous chromophores, i.e. autofluorescence, to correct for a variety of parameters affecting the magnitude of the measured signals. By doing so it also minimizes the range of baseline measurements prior to exogenous drug injection, for various tissue types. Design of the FDS and results of its testing in animals and patients using the second generation photosensitizer Tin ethyletiopurpurin (SnET2) are presented. These results support the feasibility and usefulness of the Ratio FDS system.

Paper Details

Date Published: 1 May 1995
PDF: 11 pages
Proc. SPIE 2396, Biomedical Optoelectronic Instrumentation, (1 May 1995); doi: 10.1117/12.208418
Show Author Affiliations
Daniel R. Doiron, PDT Systems, Inc. (United States)
J. Brian Dunn, PDT Systems, Inc. (United States)
W. L. Mitchell, PDT Systems, Inc. (United States)
Brian K. Dalton, PDT Systems, Inc. (United States)
Greta M. Garbo, PDT Pharmaceuticals, Inc. (United States)
Jon A. Warner, PDT Systems, Inc. (United States)


Published in SPIE Proceedings Vol. 2396:
Biomedical Optoelectronic Instrumentation
James A. Harrington; David M. Harris; Abraham Katzir, Editor(s)

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