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Proceedings Paper

Fluorescence characterization of water-driven self-assembled lipids and their temperature-induced phase transitions
Author(s): N. Idayu Zahid; Osama K. Abou-Zied; Rauzah Hashim
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Paper Abstract

Water-driven self-assembly of lipids displays a variety of liquid crystalline phases that are crucial for membrane functions. In this work, we characterized the temperature-induced phase transitions in aqueous self-assembly systems using steady-state and time-resolved fluorescence measurements. The polar head group region was investigated using tryptophan (Trp) and two of its ester derivatives, and the hydrophobic tail region was probed using pyrene. The spectral changes in tryptophan and pyrene were used as a benchmark to estimate the polarity of the head group region and the tail region, respectively. A basic medium was detected and estimated for the polar region in the inverse cubic phase of a Guerbet glycolipid and was attributed to the structural effect of the narrow nanochannels. All the studied lipid compositions show completely reversible temperature-induced phase transitions, reflecting the thermodynamic equilibrium structures of their mesophases. The results reveal a large degree of heterogeneity and flexibility of the lipid self-assembly which may be crucial for carrying out different biological functions.

Paper Details

Date Published: 12 March 2015
PDF: 13 pages
Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390L (12 March 2015); doi: 10.1117/12.2083677
Show Author Affiliations
N. Idayu Zahid, Univ. of Malaya (Malaysia)
Osama K. Abou-Zied, Sultan Qaboos Univ. (Oman)
Rauzah Hashim, Univ. of Malaya (Malaysia)

Published in SPIE Proceedings Vol. 9339:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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