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Proceedings Paper

Method for accurate quantitation of background tissue optical properties in the presence of emission from a strong fluorescence marker
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Paper Abstract

Quantification of targeted fluorescence markers during neurosurgery has the potential to improve and standardize surgical distinction between normal and cancerous tissues. However, quantitative analysis of marker fluorescence is complicated by tissue background absorption and scattering properties. Correction algorithms that transform raw fluorescence intensity into quantitative units, independent of absorption and scattering, require a paired measurement of localized white light reflectance to provide estimates of the optical properties. This study focuses on the unique problem of developing a spectral analysis algorithm to extract tissue absorption and scattering properties from white light spectra that contain contributions from both elastically scattered photons and fluorescence emission from a strong fluorophore (i.e. fluorescein). A fiber-optic reflectance device was used to perform measurements in a small set of optical phantoms, constructed with Intralipid (1% lipid), whole blood (1% volume fraction) and fluorescein (0.16-10 μg/mL). Results show that the novel spectral analysis algorithm yields accurate estimates of tissue parameters independent of fluorescein concentration, with relative errors of blood volume fraction, blood oxygenation fraction (BOF), and the reduced scattering coefficient (at 521 nm) of <7%, <1%, and <22%, respectively. These data represent a first step towards quantification of fluorescein in tissue in vivo.

Paper Details

Date Published: 4 March 2015
PDF: 6 pages
Proc. SPIE 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications, 931113 (4 March 2015); doi: 10.1117/12.2083603
Show Author Affiliations
Jaime Bravo, Thayer School of Engineering at Dartmouth (United States)
Scott C. Davis, Thayer School of Engineering at Dartmouth (United States)
Norris Cotton Cancer Ctr., Dartmouth Hitchcock Medical Ctr. (United States)
David W. Roberts, Dartmouth Hitchcock Medical Ctr. (United States)
Norris Cotton Cancer Ctr., Dartmouth Hitchcock Medical Ctr. (United States)
Keith D. Paulsen, Thayer School of Engineering at Dartmouth (United States)
Norris Cotton Cancer Ctr., Dartmouth Hitchcock Medical Ctr. (United States)
Stephen Chad Kanick, Thayer School of Engineering at Dartmouth (United States)
Norris Cotton Cancer Ctr., Dartmouth Hitchcock Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 9311:
Molecular-Guided Surgery: Molecules, Devices, and Applications
Brian W. Pogue; Sylvain Gioux, Editor(s)

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