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Proceedings Paper

The effect of laser irradiation on living cells incubated with gold nanoparticles
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Paper Abstract

Plasmon-resonant nanoparticles attached to cell membranes, under laser treatment can temporarily increase membrane permeability. In this paper, the influence of continuous-wave and pulsed (nanosecond) laser irradiation on living cells incubated with gold nanoparticles was investigated. Gold nanospheres, nanostars, and nanorods with different functionalization were used as plasmonic agents. The dependence between increase of medium temperature on the irradiation time was showed for nanostars and nanorods with different surface properties. Cells samples incubated with gold nanorods showed the highest temperature increase. Feasibility of cell optoporation by the use of gold nanospheres with variable functionalization was demonstrated. The cell membrane permeability was successfully enhanced as shown by the uptake of the fluorescent dye upon nanosecond laser treatment. Toxicity of the nanoparticles was estimated by MTT assay.

Paper Details

Date Published: 11 March 2015
PDF: 9 pages
Proc. SPIE 9340, Plasmonics in Biology and Medicine XII, 934010 (11 March 2015); doi: 10.1117/12.2083023
Show Author Affiliations
Olga Bibikova, Univ. of Oulu (Finland)
Saratov State Univ. (Russian Federation)
Prateek Singh, Univ. of Oulu (Finland)
Alexey Popov, Univ. of Oulu (Finland)
Georgy Akchurin, Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Russian Federation)
Ilya Skovorodkin, Univ. of Oulu (Finland)
Vitaly Khanadeev, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Saratov State Univ. (Russian Federation)
Matti Kinnunen, Univ. of Oulu (Finland)
Vladimir Bogatyrev, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Saratov State Univ. (Russian Federation)
Nikolai Khlebtsov, Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)
Saratov State Univ. (Russian Federation)
Seppo Vainio, Univ. of Oulu (Finland)
Valery Tuchin, Univ. of Oulu (Russian Federation)
Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Finland)


Published in SPIE Proceedings Vol. 9340:
Plasmonics in Biology and Medicine XII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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