Share Email Print
cover

Proceedings Paper

Three dimensional time-gated tracking of non-blinking quantum dots in live cells
Author(s): Matthew S. DeVore; Dominik G. Stich; Aaron M. Keller; Yagna Ghosh; Peter M. Goodwin; Mary E. Phipps; Michael H. Stewart; Cédric Cleyrat; Bridget S. Wilson; Diane S. Lidke; Jennifer A. Hollingsworth; James H. Werner
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

Paper Details

Date Published: 12 March 2015
PDF: 15 pages
Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 933812 (12 March 2015); doi: 10.1117/12.2082943
Show Author Affiliations
Matthew S. DeVore, Los Alamos National Lab. (United States)
Dominik G. Stich, Los Alamos National Lab. (United States)
Aaron M. Keller, Los Alamos National Lab. (United States)
Yagna Ghosh, Los Alamos National Lab. (United States)
Peter M. Goodwin, Los Alamos National Lab. (United States)
Mary E. Phipps, Los Alamos National Lab. (United States)
Michael H. Stewart, U.S. Naval Research Lab. (United States)
Cédric Cleyrat, The Univ. of New Mexico (United States)
Bridget S. Wilson, The Univ. of New Mexico (United States)
Diane S. Lidke, The Univ. of New Mexico (United States)
Jennifer A. Hollingsworth, Los Alamos National Lab. (United States)
James H. Werner, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 9338:
Colloidal Nanoparticles for Biomedical Applications X
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

© SPIE. Terms of Use
Back to Top