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Proceedings Paper

An imaging and analysis toolset for the study of Caenorhabditis elegans neurodevelopment
Author(s): Ryan Christensen; Alexandra Bokinsky; Anthony Santella; Yicong Wu; Javier Marquina; Ismar Kovacevic; Abhishek Kumar; Peter Winter; Evan McCreedy; William Mohler; Zhirong Bao; Daniel Colón-Ramos; Hari Shroff
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Paper Abstract

How an entire nervous system develops remains mysterious. We have developed a light-sheet microscope system to examine neurodevelopment in C. elegans embryos. Our system creates overlapping light sheets from two orthogonally positioned objectives, enabling imaging from the first cell division to hatching (~14 hours) with 350 nm isotropic resolution. We have also developed computer algorithms to computationally straighten nematode embryos, facilitating data comparison and combination from multiple animals. We plan to use these tools to create an atlas showing the position and morphology of all neurons in the developing embryo.

Paper Details

Date Published: 10 March 2015
PDF: 9 pages
Proc. SPIE 9334, Optical Methods in Developmental Biology III, 93340C (10 March 2015); doi: 10.1117/12.2082394
Show Author Affiliations
Ryan Christensen, National Institutes of Health (United States)
Alexandra Bokinsky, National Institutes of Health (United States)
Anthony Santella, Sloan-Kettering Institute (United States)
Yicong Wu, National Institutes of Health (United States)
Javier Marquina, Yale Univ. School of Medicine (United States)
Ismar Kovacevic, Sloan-Kettering Institute (United States)
Abhishek Kumar, National Institutes of Health (United States)
Yale Univ. School of Medicine (United States)
Peter Winter, National Institutes of Health (United States)
Evan McCreedy, National Institutes of Health (United States)
William Mohler, Univ. of Connecticut Health Ctr. (United States)
Zhirong Bao, Sloan-Kettering Institute (United States)
Daniel Colón-Ramos, Yale Univ. School of Medicine (United States)
Hari Shroff, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 9334:
Optical Methods in Developmental Biology III
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma, Editor(s)

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