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Proceedings Paper

A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay
Author(s): N. Punjabi; Jitendra Satija; S. Mukherji
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Paper Abstract

In this paper, we propose a novel „gold on gold‟ biosensing scheme for absorbance based fiber-optic biosensor. First, a self-assembled monolayer of gold nanoparticles is formed at the sensing region of the fiber-optic probe by incubating an amino-silanized probe in a colloidal gold solution. Thereafter, the receptor moieties, i.e. Human immunoglobulin G (HIgG) were immobilized by using standard alkanethiol and classic carbodiimide coupling chemistry. Finally, biosensing experiments were performed with different concentrations of gold nanoparticle-tagged analyte, i.e. Goat anti- Human immunoglobulin G (Nanogold-GaHIgG). The sensor response was observed to be more than five-fold compared to the control bioassay, in which the sensor matrix was devoid of gold nanoparticle film. Also, the response was found to be ~10 times higher compared to the FITC-tagged scheme and ~14.5 times better compared to untagged scheme. This novel scheme also demonstrated the potential in improving the limit of detection for the fiber-optic biosensors.

Paper Details

Date Published: 5 May 2015
PDF: 7 pages
Proc. SPIE 9506, Optical Sensors 2015, 95062H (5 May 2015); doi: 10.1117/12.2081233
Show Author Affiliations
N. Punjabi, Indian Institute of Technology Bombay (India)
Jitendra Satija, Vellore Institute of Technology (India)
S. Mukherji, Indian Institute of Technology Bombay (India)


Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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