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Proceedings Paper

Light shift from ultraviolet to near infrared light: Cerenkov luminescence with gold nanocluster – near infrared (AuNc-NIR) conjugates
Author(s): Su Woong Yoo; Hyoyoung Mun; Gyungseok Oh; Youngjae Ryu; Min-Gon Kim; Euiheon Chung
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Paper Abstract

Cerenkov luminescence (CL) is generated when a charged particle moves faster than the speed of light in dielectric media. Recently CL imaging becomes an emerging technique with the use of radioisotopes. However, due to relatively weak blue light production and massive tissue attenuation, CL has not been applied widely. Therefore, we attempted to shift the CL emission to more near infrared (NIR) spectrum for better tissue penetration by using Cerenkov Radiation Energy Transfer (CRET). Gold nanoclusters were conjugated with NIR dye molecules (AuNc-IR820 and AuNc-ICG) to be activated with ultraviolet light. We found optimal conjugate concentrations of AuNc-NIR conjugates by spectroscopy system to generate maximal photon emission. When exposed by ultraviolet light, the emission of NIR light from the conjugates were verified. In quantitative analysis, AuNc-NIR conjugates emit brighter light signal than pure AuNc. This result implies that NIR fluorescent dyes (both IR820 and ICG) can be excited by the emission from AuNc. Following the above baseline experiment, we mixed F-18 fluorodeoxyglucose (F-18 FDG) radioisotope to the AuNc- NIR conjugates, to confirm NIR emission induced from Cerenkov radiation. Long pass filter was used to block Cerenkov luminescence and to collect the emission from AuNc-NIR conjugates. Instead of one long exposure imaging with CCD, we used multiple frame scheme to eliminate gamma radiation strike in each frame prior to combination. In summary, we obtained NIR emission light from AuNc-NIR conjugated dyes that is induced from CL. We plan to perform in vivo small animal imaging with these conjugates to assess better tissue penetration.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280C (2 March 2015); doi: 10.1117/12.2080898
Show Author Affiliations
Su Woong Yoo, Gwangju Institute of Science and Technology (Korea, Republic of)
Hyoyoung Mun, Gwangju Institute of Science and Technology (Korea, Republic of)
Gyungseok Oh, Gwangju Institute of Science and Technology (Korea, Republic of)
Youngjae Ryu, Gwangju Institute of Science and Technology (Korea, Republic of)
Min-Gon Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Euiheon Chung, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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