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Proceedings Paper

Molecular effective coverage surface area of optical clearing agents for predicting optical clearing potential
Author(s): Wei Feng; Ning Ma; Dan Zhu
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Paper Abstract

The improvement of methods for optical clearing agent prediction exerts an important impact on tissue optical clearing technique. The molecular dynamic simulation is one of the most convincing and simplest approaches to predict the optical clearing potential of agents by analyzing the hydrogen bonds, hydrogen bridges and hydrogen bridges type forming between agents and collagen. However, the above analysis methods still suffer from some problem such as analysis of cyclic molecule by reason of molecular conformation. In this study, a molecular effective coverage surface area based on the molecular dynamic simulation was proposed to predict the potential of optical clearing agents. Several typical cyclic molecules, fructose, glucose and chain molecules, sorbitol, xylitol were analyzed by calculating their molecular effective coverage surface area, hydrogen bonds, hydrogen bridges and hydrogen bridges type, respectively. In order to verify this analysis methods, in vitro skin samples optical clearing efficacy were measured after 25 min immersing in the solutions, fructose, glucose, sorbitol and xylitol at concentration of 3.5 M using 1951 USAF resolution test target. The experimental results show accordance with prediction of molecular effective coverage surface area. Further to compare molecular effective coverage surface area with other parameters, it can show that molecular effective coverage surface area has a better performance in predicting OCP of agents.

Paper Details

Date Published: 13 March 2015
PDF: 8 pages
Proc. SPIE 9322, Dynamics and Fluctuations in Biomedical Photonics XII, 932216 (13 March 2015); doi: 10.1117/12.2080643
Show Author Affiliations
Wei Feng, Huazhong Univ. of Science and Technology (China)
Ning Ma, Huazhong Univ. of Science and Technology (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 9322:
Dynamics and Fluctuations in Biomedical Photonics XII
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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