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Proceedings Paper

Excitation light leakage suppression using temperature sensitive fluorescent agents
Author(s): Farouk Nouizi; Tiffany C. Kwong; Jessica Kwong; Jaedu Cho; Yuwen Chan; Uma Sampathkumaran; Yue Zhu; Maksudul M. Alam; Gultekin Gulsen
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Paper Abstract

Fluorescence tomography is a non invasive, non ionizing imaging technique able to provide a 3D distribution of fluorescent agents within thick highly scattering mediums, using low cost instrumentation. However, its low spatial resolution due to undetermined and ill-posed nature of its inverse problem has delayed its integration into the clinical settings. In addition, the quality of the fluorescence tomography images is degraded due to the excitation light leakage contaminating the fluorescence measurements. This excitation light leakage results from the excitation photons that cannot be blocked by the fluorescence filters. In this contribution, we present a new method to remove this excitation light leakage noise based on the use of a temperature sensitive fluorescence agents. By performing different sets of measurements using this temperature sensitive agents at multiple temperatures, the excitation light leakage can be estimated and then removed from the measured fluorescence signals . The results obtained using this technique demonstrate its potential for use in in-vivo small animal imaging.

Paper Details

Date Published: 12 March 2015
PDF: 5 pages
Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93190Y (12 March 2015); doi: 10.1117/12.2080630
Show Author Affiliations
Farouk Nouizi, Univ. of California, Irvine (United States)
Tiffany C. Kwong, Univ. of California, Irvine (United States)
Jessica Kwong, Univ. of California, Irvine (United States)
Jaedu Cho, Univ. of California, Irvine (United States)
Yuwen Chan, Univ. of California, Irvine (United States)
Uma Sampathkumaran, InnoSense LLC (United States)
Yue Zhu, InnoSense LLC (United States)
Maksudul M. Alam, InnoSense LLC (United States)
Gultekin Gulsen, Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 9319:
Optical Tomography and Spectroscopy of Tissue XI
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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