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Proceedings Paper

Label-free analysis of single and multiple cells with a 2D light scattering static cytometer
Author(s): Shanshan Liu; Linyan Xie; Yan Yang; Xu Qiao; Kun Song; Beihua Kong; Xuantao Su
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Paper Abstract

Cytometry has wide applications in biomedicine for cell differentiation or disease monitoring. Here we report our newly developed two dimensional (2D) light scattering static cytometric technique for single and multiple cell analysis. The static cytometer adopts a scanning fiber probe for cell excitation and obtains 2D light scattering patterns on a complementary metal oxide semiconductor (CMOS) detector. Our results show that experimental 2D light scattering patterns obtained from single yeast cells are with fringe structure while those from multiple yeast cells give speckle patterns. The experimental results compare favorably with our 2D light scattering Mie theory simulations for both single and multiple cells. The varying of 2D light scattering patterns with different yeast cell clusters, either number or distribution changes, shows the potential of our 2D light scattering static cytometer for cellular diagnostics.

Paper Details

Date Published: 2 March 2015
PDF: 5 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281B (2 March 2015); doi: 10.1117/12.2080519
Show Author Affiliations
Shanshan Liu, Shandong Univ. (China)
Linyan Xie, Shandong Univ. (China)
Yan Yang, Shandong Univ. (China)
Xu Qiao, Shandong Univ. (China)
Kun Song, Qilu Hospital of Shandong Univ. (China)
Beihua Kong, Qilu Hospital of Shandong Univ. (China)
Xuantao Su, Shandong Univ. (China)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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