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Proceedings Paper

An automated tool for 3D tracking of single molecules in living cells
Author(s): L. Gardini; M. Capitanio; F. S. Pavone
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Paper Abstract

Since the behaviour of proteins and biological molecules is tightly related to cell’s environment, more and more microscopy techniques are moving from in vitro to in living cells experiments. Looking at both diffusion and active transportation processes inside a cell requires three-dimensional localization over a few microns range, high SNR images and high temporal resolution. Since protein dynamics inside a cell involve all three dimensions, we developed an automated routine for 3D tracking of single fluorescent molecules inside living cells with nanometer accuracy, by exploiting the properties of the point-spread-function of out-of-focus Quantum Dots bound to the protein of interest.

Paper Details

Date Published: 9 March 2015
PDF: 6 pages
Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933117 (9 March 2015); doi: 10.1117/12.2080378
Show Author Affiliations
L. Gardini, European Lab. for Non-linear Spectroscopy (Italy)
M. Capitanio, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
F. S. Pavone, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Instituto Nazionale di Ottica (Italy)


Published in SPIE Proceedings Vol. 9331:
Single Molecule Spectroscopy and Superresolution Imaging VIII
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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