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Proceedings Paper

Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers
Author(s): Carina Monico; Alessia Tempestini; Francesco Vanzi; Francesco S. Pavone; Marco Capitanio
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Paper Abstract

The lac operon is a well-known example of gene expression regulation, based on the specific interaction of Lac repressor protein (LacI) with its target DNA sequence (operator). We recently developed an ultrafast force-clamp laser trap technique capable of probing molecular interactions with sub-ms temporal resolution, under controlled pN-range forces. With this technique, we tested the interaction of LacI with different DNA constructs. Based on position along the DNA sequence, the observed interactions can be interpreted as specific binding to operator sequences and transient interactions with nonspecific sequences.

Paper Details

Date Published: 9 March 2015
PDF: 6 pages
Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933116 (9 March 2015); doi: 10.1117/12.2080347
Show Author Affiliations
Carina Monico, Univ. of Oxford (United Kingdom)
Alessia Tempestini, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Francesco Vanzi, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Francesco S. Pavone, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
National Institute of Optics, National Research Council (Italy)
Marco Capitanio, European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 9331:
Single Molecule Spectroscopy and Superresolution Imaging VIII
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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