Share Email Print
cover

Proceedings Paper

Realtime wavefront sensing in a SPIM microscope, and active aberration tracking
Author(s): Jonathan M. Taylor; Christopher D. Saunter; Cyril Bourgenot; John M. Girkin; Gordon D. Love
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Paper Details

Date Published: 31 March 2015
PDF: 6 pages
Proc. SPIE 9335, Adaptive Optics and Wavefront Control for Biological Systems, 93350A (31 March 2015); doi: 10.1117/12.2080061
Show Author Affiliations
Jonathan M. Taylor, Univ. of Glasgow (United Kingdom)
Christopher D. Saunter, Durham Univ. (United Kingdom)
Cyril Bourgenot, Durham Univ. (United Kingdom)
John M. Girkin, Durham Univ. (United Kingdom)
Gordon D. Love, Durham Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 9335:
Adaptive Optics and Wavefront Control for Biological Systems
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

© SPIE. Terms of Use
Back to Top