Share Email Print
cover

Proceedings Paper

Molecular constituents of colorectal cancer metastatic to the liver by imaging infrared spectroscopy
Author(s): James V. Coe; Zhaomin Chen; Ran Li; Steven V Nystrom; Ryan Butke; Barrie Miller; Charles L. Hitchcock; Heather C. Allen; Stephen P. Povoski; Edward W. Martin
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Infrared (IR) imaging spectroscopy of human liver tissue slices has been used to identify and characterize liver metastasis of colorectal origin which was surgically removed from a consenting patient and frozen without formalin fixation or dehydration procedures, so that lipids and water remain in the tissues. First, a k-means clustering analysis, using metrics from the IR spectra, identified groups within the image. The groups were identified as tumor or nontumor regions by comparing to an H and E stain of the same sample after IR imaging. Then, calibrant IR spectra of protein, several fats, glycogen, and polyvinyl alcohol were isolated by differencing spectra from different regions or groups in the image space. Finally, inner products (or scores) of the IR spectra at each pixel in the image with each of the various calibrants were calculated showing how the calibrant molecules vary in tumor and nontumor regions. In this particular case, glycogen and protein changes enable separation of tumor and nontumor regions as shown with a contour plot of the glycogen scores versus the protein scores.

Paper Details

Date Published: 2 March 2015
PDF: 7 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280R (2 March 2015); doi: 10.1117/12.2079884
Show Author Affiliations
James V. Coe, The Ohio State Univ. (United States)
Zhaomin Chen, The Ohio State Univ. (United States)
Ran Li, The Ohio State Univ. (United States)
Steven V Nystrom, The Ohio State Univ. (United States)
Ryan Butke, The Ohio State Univ. (United States)
Barrie Miller, The Ohio State Univ. (United States)
Charles L. Hitchcock, The Ohio State Univ. (United States)
Heather C. Allen, The Ohio State Univ. (United States)
Stephen P. Povoski, The Ohio State Univ. (United States)
Edward W. Martin, The Ohio State Univ. (United States)


Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top