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Proceedings Paper

Hyperspectral imaging to monitor simultaneously multiple protein subtypes and live track their spatial dynamics: a new platform to screen drugs for CNS diseases
Author(s): S. Labrecque; J.-P. Sylvestre; S. Marcet; Francesca Mangiarini; M. Verhaegen; P. De Koninck; S. Blais-Ouellette
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Paper Abstract

In the past decade, the efficacy of existing therapies and the discovery of innovative treatments for Central Nervous System (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. In order to better understand the fundamental mechanisms that regulate diseases of the CNS, a fast fluorescence hyperspectral imaging platform was designed to track simultaneously various neurotransmitter receptors trafficking in and out of synapses. With this hyperspectral imaging platform, it was possible to image simultaneously five different synaptic proteins, including subtypes of glutamate receptors (mGluR, NMDAR, AMPAR), postsynaptic density proteins, and signaling proteins. This new imaging platform allows fast simultaneous acquisitions of at least five fluorescent markers in living neurons with a high spatial resolution. This technique provides an effective method to observe several synaptic proteins at the same time, thus study how drugs for CNS impact the spatial dynamics of these proteins.

Paper Details

Date Published: 2 March 2015
PDF: 7 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280L (2 March 2015); doi: 10.1117/12.2079882
Show Author Affiliations
S. Labrecque, Institut Univ. en santé mental de Québec (Canada)
J.-P. Sylvestre, Photon etc. Inc. (Canada)
S. Marcet, Photon etc. Inc. (Canada)
Francesca Mangiarini, Photon etc. Inc. (Canada)
M. Verhaegen, Photon etc. Inc. (Canada)
P. De Koninck, Institut Univ. en santé mental de Québec (Canada)
S. Blais-Ouellette, Photon etc. Inc. (Canada)


Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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