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Proceedings Paper

Multi-modal contrast of tissue anatomy enables correlative biomarker imaging
Author(s): Karl Garsha; Franklin Ventura; Gary Pestano; Michael Otter; Dea Nagy; Ray B. Nagle; Esteban Roberts; Michael Barnes
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Paper Abstract

Optical imaging techniques are being developed that promise to increase the information content related to specific molecular reporters. Such modalities do not produce contrast in the structural context of the surrounding tissue, making it difficult to reconcile molecular information with morphological context. We report a solution that enables visualization of the tissue morphology on formalin-fixed, paraffin embedded sections prepared for analytical biomarker imaging. Our approach combines modes of transmitted darkfield and fluorescence contrast and computer visualization to produce 2-component image data analogous to the classical hematoxylin and eosin histological stain. An interferometric hyperspectral image capture mode enables measurement of multiplexed biomarkers in annotated anatomic regions. The system enables practical correlative analysis of molecular changes within areas of anatomic pathology.

Paper Details

Date Published: 5 March 2015
PDF: 15 pages
Proc. SPIE 9316, Multimodal Biomedical Imaging X, 931608 (5 March 2015); doi: 10.1117/12.2079872
Show Author Affiliations
Karl Garsha, Ventana Medical Systems, Inc. (United States)
Franklin Ventura, Ventana Medical Systems, Inc. (United States)
Gary Pestano, Ventana Medical Systems, Inc. (United States)
Michael Otter, Ventana Medical Systems, Inc. (United States)
Dea Nagy, Ventana Medical Systems, Inc. (United States)
Ray B. Nagle, The Univ. of Arizona (United States)
Esteban Roberts, Ventana Medical Systems, Inc. (United States)
Michael Barnes, Ventana Medical Systems, Inc. (United States)


Published in SPIE Proceedings Vol. 9316:
Multimodal Biomedical Imaging X
Fred S. Azar; Xavier Intes, Editor(s)

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