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Proceedings Paper

Early detection of melanoma with the combined use of acoustic microscopy, infrared reflectance and Raman spectroscopy
Author(s): Georgios T. Karagiannis; Giannis Grivas; Anastasia Tsingotjidou; Georgios K. Apostolidis; Ifigeneia Grigoriadou; I. Dori; Kyriaki-Nefeli Poulatsidou; Argyrios Doumas; Stefan Wesarg; Panagiotis Georgoulias
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Paper Abstract

Malignant melanoma is a form of skin cancer, with increasing incidence worldwide. Early diagnosis is crucial for the prognosis and treatment of the disease. The objective of this study is to develop a novel animal model of melanoma and apply a combination of the non-invasive imaging techniques acoustic microscopy, infrared (IR) and Raman spectroscopies, for the detection of developing tumors. Acoustic microscopy provides information about the 3D structure of the tumor, whereas, both spectroscopic modalities give qualitative insight of biochemical changes during melanoma development. In order to efficiently set up the final devices, propagation of ultrasonic and electromagnetic waves in normal skin and melanoma simulated structures was performed. Synthetic and grape-extracted melanin (simulated tumors), endermally injected, were scanned and compared to normal skin. For both cases acoustic microscopy with central operating frequencies of 110MHz and 175MHz were used, resulting to the tomographic imaging of the simulated tumor, while with the spectroscopic modalities IR and Raman differences among spectra of normal and melanin- injected sites were identified in skin depth. Subsequently, growth of actual tumors in an animal melanoma model, with the use of human malignant melanoma cells was achieved. Acoustic microscopy and IR and Raman spectroscopies were also applied. The development of tumors at different time points was displayed using acoustic microscopy. Moreover, the changes of the IR and Raman spectra were studied between the melanoma tumors and adjacent healthy skin. The most significant changes between healthy skin and the melanoma area were observed in the range of 900-1800cm-1 and 350-2000cm-1, respectively.

Paper Details

Date Published: 11 March 2015
PDF: 9 pages
Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93232T (11 March 2015); doi: 10.1117/12.2079690
Show Author Affiliations
Georgios T. Karagiannis, Univ. of Thessaly (Greece)
Giannis Grivas, Aristotle Univ. of Thessaloniki (Greece)
Anastasia Tsingotjidou, Aristotle Univ. of Thessaloniki (Greece)
Georgios K. Apostolidis, Ormylia Foundation (Greece)
Ifigeneia Grigoriadou, ORMYLIA Art Diagnosis Ctr. (Greece)
I. Dori, Aristotle Univ. of Thessaloniki (Greece)
Kyriaki-Nefeli Poulatsidou, Aristotle Univ. of Thessaloniki (Greece)
Argyrios Doumas, Aristotle Univ. of Thessaloniki (Greece)
Stefan Wesarg, Fraunhofer-Institut für Graphische Datenverarbeitung (Germany)
Panagiotis Georgoulias, Univ. of Thessaly (Greece)


Published in SPIE Proceedings Vol. 9323:
Photons Plus Ultrasound: Imaging and Sensing 2015
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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