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Proceedings Paper

Low level light in combination with metabolic modulators for effective therapy
Author(s): Tingting Dong; Qi Zhang; Michael R. Hamblin; Mei X. Wu
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Paper Abstract

Vascular damage occurs frequently at the injured brain causing hypoxia and is associated with poor outcomes in the clinics. We found high levels of glycolysis, reduced ATP generation, and increased formation of reactive oxygen species (ROS) and apoptosis in neurons under hypoxia. Strikingly, these adverse events were reversed significantly by noninvasive exposure of injured brain to low-level light (LLL). LLL illumination sustained the mitochondrial membrane potential, constrained cytochrome C leakage in hypoxic cells, and protected them from apoptosis, underscoring a unique property of LLL. The effect of LLL was further bolstered by combination with metabolic substrates such as pyruvate or lactate both in vivo and in vitro. The combinational treatment retained memory and learning activities of injured mice to a normal level, whereas those treated with LLL or pyruvate alone, or sham light displayed partial or severe deficiency in these cognitive functions. In accordance with well-protected learning and memory function, the hippocampal region primarily responsible for learning and memory was completely protected by a combination of LLL and pyruvate, in marked contrast to the severe loss of hippocampal tissue due to secondary damage in control mice. These data clearly suggest that energy metabolic modulators can additively or synergistically enhance the therapeutic effect of LLL in energy-producing insufficient tissues like injured brain. Keywords:

Paper Details

Date Published: 5 March 2015
PDF: 22 pages
Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090A (5 March 2015); doi: 10.1117/12.2079363
Show Author Affiliations
Tingting Dong, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Qi Zhang, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Michael R. Hamblin, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Mei X. Wu, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 9309:
Mechanisms for Low-Light Therapy X
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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