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Proceedings Paper

Interstitial photodynamic therapy and glioblastoma: light fractionation study on a preclinical model: preliminary results
Author(s): Henri-Arthur Leroy; Maximilien Vermandel; Marie-Charlotte Tétard; Jean-Paul Lejeune; Serge Mordon; Nicolas Reyns
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Paper Abstract

Background
Glioblastoma is a high-grade cerebral tumor with local recurrence and poor outcome. Photodynamic therapy (PDT) is a local treatment based on the light activation of a photosensitizer (PS) in the presence of oxygen to form cytotoxic species. Fractionation of light delivery may enhance treatment efficiency by restoring tissue oxygenation.

Objectives
To evaluate the efficiency of light fractionation using MRI imaging, including diffusion and perfusion, compared to histological data.

Materials and Methods
Thirty-nine “Nude” rats were grafted with human U87 cells into the right putamen. After PS precursor intake (5-ALA), an optic fiber was introduced into the tumor. The rats were randomized in three groups: without illumination, with monofractionated illumination and the third one with multifractionated light. Treatment effects were assessed with early MRI including diffusion and perfusion sequences. The animals were eventually sacrificed to perform brain histology.

Results
On MRI, we observed elevated diffusion values in the center of the tumor among treated animals, especially in multifractionated group. Perfusion decreased around the treatment site, all the more in the multifractionated group. Histology confirmed our MRI findings, with a more extensive necrosis and associated with a rarified angiogenic network in the treatment area, after multifractionated PDT. However, we observed more surrounding edema and neovascularization in the peripheral ring after multifractionated PDT.

Conclusion
Fractionated interstitial PDT induced specific tumoral lesions. The multifractionated scheme was more efficient, inducing increased tumoral necrosis, but it also caused significant peripheral edema and neovascularization. Diffusion and perfusion MRI imaging were able to predict the histological lesions.

Paper Details

Date Published: 10 March 2015
PDF: 17 pages
Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93050D (10 March 2015); doi: 10.1117/12.2079347
Show Author Affiliations
Henri-Arthur Leroy, INSERM (France)
Lille Univ. Hospital (France)
Univ. of Lille 2 (France)
Maximilien Vermandel, INSERM (France)
Lille Univ. Hospital (France)
Univ. of Lille 2 (France)
Marie-Charlotte Tétard, INSERM (France)
Lille Univ. Hospital (France)
Univ. of Lille 2 (France)
Jean-Paul Lejeune, INSERM (France)
Lille Univ. Hospital (France)
Univ. of Lille 2 (France)
Serge Mordon, INSERM (France)
Nicolas Reyns, INSERM (France)
Lille Univ. Hospital (France)
Univ. of Lille 2 (France)


Published in SPIE Proceedings Vol. 9305:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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