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Proceedings Paper

Effectiveness of antimicrobial photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser
Author(s): Juliana S. C. Monteiro; Susana C. P. S. de Oliveira; Gustavo M. Pires-Santos; Fernando José Pires Sampaio; Fátima Antônia Aparecida Zanin; Antônio L. B. Pinheiro
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Paper Abstract

The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 μg/mL) on Staphylococcus aureus (ATCC 23529) irradiated or not with the red laser (λ 660 nm, 12J/cm2). All tests were performed in triplicate and samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus.

Paper Details

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090X (5 March 2015); doi: 10.1117/12.2079170
Show Author Affiliations
Juliana S. C. Monteiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Univ. Estadual de Feira de Santana (Brazil)
Susana C. P. S. de Oliveira, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Gustavo M. Pires-Santos, Ctr. de Pesquisas Gonçalo Muniz, Fundação Oswaldo Cruz (Brazil)
Fernando José Pires Sampaio, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Fátima Antônia Aparecida Zanin, National Institute of Optics and Photonics (Brazil)
Brugnera & Zanin Institute (Brazil)
Antônio L. B. Pinheiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Brugnera & Zanin Institute (Brazil)


Published in SPIE Proceedings Vol. 9309:
Mechanisms for Low-Light Therapy X
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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