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Proceedings Paper

Evaluation of the efficacy of photodynamic antimicrobial therapy using a phenothiazine compound and Laser (l=660hm) on the interface: macrophage vs S. aureus
Author(s): Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Gustavo M. Pires-Santos; Fernando José Pires Sampaio; Fátima Antônia Aparecida Zanin; Antônio L. B. Pinheiro
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Paper Abstract

Nowadays photodynamic inactivation has been proposed as an alternative treatment for localized bacterial infections as a response to the problem of antibiotic resistance. Much is already known about the photodynamic inactivation of microorganisms: both antibiotic-sensitive and -resistant strains can be successfully photoinactivated and there is the additional advantage that repeated photosensitization of bacterial cells does not induce a selection of resistant strains. Staphylococcus spp. are opportunistic microorganisms known for their capacity to develop resistance against antimicrobial agents. The emergence of resistant strains of bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) poses a major challenge to healthcare. MRSA is a major cause of hospital-acquired infection throughout the world and is now also prevalent in the community as well as nursing and residential homes. The aim of this study was to evaluate the phagocytic function of macrophages J774 against S. aureus in the presence and absence of AmPDT with phenothiazine compound (12.5 μg/mL) and low level laser (λ=660nm, 12 J/cm²). Experimental groups: Control group (L-P-), Phenothiazine group (L-P+) Laser group (L+P-), AmPDT group (L+P+).The tests presented in this study were performed in triplicate. This study showed that AmPDT induced bacterial death in about 80% as well as increasing phagocytic capacity of macrophages by approximately 20% and enhanced the antimicrobial activity by approximately 50% compared to the control group and enabling more intense oxidative burst.

Paper Details

Date Published: 5 March 2015
PDF: 8 pages
Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090N (5 March 2015); doi: 10.1117/12.2079134
Show Author Affiliations
Susana C. P. S. de Oliveira, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Juliana S. C. Monteiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Univ. Estadual de Feira de Santana (Brazil)
Gustavo M. Pires-Santos, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Fernando José Pires Sampaio, Univ. Federal da Bahia (Brazil)
Fátima Antônia Aparecida Zanin, National Institute of Optics and Photonics (Brazil)
Brugnera & Zanin Institute (Brazil)
Antônio L. B. Pinheiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)


Published in SPIE Proceedings Vol. 9309:
Mechanisms for Low-Light Therapy X
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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