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Proceedings Paper

Non-linear fluorescence excitation of Rhodamine 6G and TRITC labeled IgG in whispering gallery mode microresonators
Author(s): Carme Pastells; M. Pilar Marco; David Merino-Arranz; Pablo Loza-Alvarez; Laura Pasquardini; Cecilia Pederzolli; Daniele Farnesi; Simone Berneschi; Giancarlo C. Righini; Gualtiero Nunzi Conti; Silvia Soria Huguet
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Paper Abstract

We report the non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a microspherical whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also tested the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are tetramethyl rhodamine isothiocyanate and Rhodamine 6G. All measurements were performed in a modified confocal microscope.

Paper Details

Date Published: 3 March 2015
PDF: 9 pages
Proc. SPIE 9343, Laser Resonators, Microresonators, and Beam Control XVII, 93430K (3 March 2015); doi: 10.1117/12.2079112
Show Author Affiliations
Carme Pastells, Nanobiotechnology for Diagnostics group (Nb4Dg), IQAC-CSIS (Spain)
CIBER de Bioingeniería, Biomateriales y Nanomedicina (Spain)
M. Pilar Marco, Nanobiotechnology for Diagnostics group (Nb4Dg), IQAC-CSIS (Spain)
CIBER de Bioingeniería, Biomaterials y Nanomedicina (Spain)
David Merino-Arranz, ICFO - Institut de Ciències Fotòniques (Spain)
Pablo Loza-Alvarez, ICFO - Institut de Ciències Fotòniques (Spain)
Laura Pasquardini, Fondazione Bruno Kessler (Italy)
Cecilia Pederzolli, Fondazione Bruno Kessler (Italy)
Daniele Farnesi, Istituto di Fisica Applicata Nello Carrara (Italy)
Museo Storico della Fisica e Centro Studi e Ricerche "E. Fermi" (Italy)
Simone Berneschi, Istituto di Fisica Applicata Nello Carrara (Italy)
Giancarlo C. Righini, Istituto di Fisica Applicata Nello Carrara (Italy)
Museo Storico della Fisica e Centro Studi e Ricerche "E. Fermi" (Italy)
Gualtiero Nunzi Conti, Istituto di Fisica Applicata Nello Carrara (Italy)
Silvia Soria Huguet, Istituto di Fisica Applicata Nello Carrara (Italy)


Published in SPIE Proceedings Vol. 9343:
Laser Resonators, Microresonators, and Beam Control XVII
Alexis V. Kudryashov; Alan H. Paxton; Vladimir S. Ilchenko; Lutz Aschke; Kunihiko Washio, Editor(s)

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