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Proceedings Paper

Cholesterol efflux monitoring in macrophage form cells by using fluorescence lifetime imaging
Author(s): Young Sik Song; Sang Hak Lee; Byoung Hee Park; Soo Hyeok Kim; Won Sang Hwang; Dug Young Kim
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Paper Abstract

Macrophages play a key role in atherosclerotic plaque destabilization and rupture, since they accumulate large amounts of lipid through the uptake of modified lipoproteins which results in foam cell formation. Cholesterol efflux is the process of removing cholesterol from macrophages in the subintima of the vessel wall, and efflux mechanism in a cell is one of the critical issues for the prevention of cardiovascular diseases. High density lipoproteins (HDL) stimulate cholesterol efflux from macrophage foam cells in the arterial wall. Radioisotope-labeled cholesterol analysis method is well known conventional method for observing cholesterol efflux. The major drawback of this method is its long and complicated process. Fluorescence intensity imaging schemes are replacing the radioisotope-labeled method in recent years for cholesterol efflux monitoring. Various spectroscopic methods are also adapted for cholesterol efflux imaging. Here we present a fluorescence lifetime imaging method for more quantitative observation of cholesterol efflux process in macrophages, which enables us to observe cholesterol level changes with various conditions. We used J774 macrophage cell and 25-NBD-cholesterol which is a famous cholesterol specific dye. Our lifetime imaging results clearly show cholesterol efflux rate very effectively. We believe that fluorescence lifetime analysis is new and very powerful for cholesterol imaging or monitoring.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 932808 (2 March 2015); doi: 10.1117/12.2078996
Show Author Affiliations
Young Sik Song, Yonsei Univ. (Korea, Republic of)
Sang Hak Lee, Yonsei Univ. College of Medicine (Korea, Republic of)
Byoung Hee Park, Yonsei Univ. College of Medicine (Korea, Republic of)
Soo Hyeok Kim, National Institute on Aging (United States)
Won Sang Hwang, Yonsei Univ. (Korea, Republic of)
Dug Young Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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