Share Email Print
cover

Proceedings Paper

Changes in chemical composition of bone matrix in ovariectomized (OVX) rats detected by Raman spectroscopy and multivariate analysis
Author(s): Yusuke Oshima; Tadahiro Iimura; Takashi Saitou; Takeshi Imamura
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Osteoporosis is a major bone disease that connotes the risk of fragility fractures resulting from alterations to bone quantity and/or quality to mechanical competence. Bone strength arises from both bone quantity and quality. Assessment of bone quality and bone quantity is important for prediction of fracture risk. In spite of the two factors contribute to maintain the bone strength, only one factor, bone mineral density is used to determine the bone strength in the current diagnosis of osteoporosis. On the other hand, there is no practical method to measure chemical composition of bone tissue including hydroxyapatite and collagen non-invasively. Raman spectroscopy is a powerful technique to analyze chemical composition and material properties of bone matrix non-invasively. Here we demonstrated Raman spectroscopic analysis of the bone matrix in osteoporosis model rat. Ovariectomized (OVX) rat was made and the decalcified sections of tibias were analyzed by a Raman microscope. In the results, Raman bands of typical collagen appeared in the obtained spectra. Although the typical mineral bands at 960 cm-1 (Phosphate) was absent due to decalcified processing, we found that Raman peak intensities of amide I and C-C stretching bands were significantly different between OVX and sham-operated specimens. These differences on the Raman spectra were statistically compared by multivariate analyses, principal component analysis (PCA) and liner discrimination analysis (LDA). Our analyses suggest that amide I and C-C stretching bands can be related to stability of bone matrix which reflects bone quality.

Paper Details

Date Published: 26 February 2015
PDF: 4 pages
Proc. SPIE 9303, Photonic Therapeutics and Diagnostics XI, 93033S (26 February 2015); doi: 10.1117/12.2078787
Show Author Affiliations
Yusuke Oshima, Ehime Univ. Graduate School of Medicine (Japan)
Ehime Univ. Hospital (Japan)
Ehime Univ. (Japan)
Tadahiro Iimura, Ehime Univ. Hospital (Japan)
Ehime Univ. (Japan)
Takashi Saitou, Ehime Univ. Graduate School of Medicine (Japan)
Ehime Univ. Hospital (Japan)
Takeshi Imamura, Ehime Univ. Graduate School of Medicine (Japan)
Ehime Univ. Hospital (Japan)
Ehime Univ. (Japan)


Published in SPIE Proceedings Vol. 9303:
Photonic Therapeutics and Diagnostics XI
Hyun Wook Kang; Brian J. F. Wong; Melissa C. Skala; Bernard Choi; Guillermo J. Tearney; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory; Mark W. Dewhirst; Justus F. Ilgner; Alfred Nuttal; Haishan Zeng; Laura Marcu; Claus-Peter Richter, Editor(s)

© SPIE. Terms of Use
Back to Top