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Proceedings Paper

Optimized optical clearing method for imaging central nervous system
Author(s): Tingting Yu; Yisong Qi; Hui Gong; Qingming Luo; Dan Zhu
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Paper Abstract

The development of various optical clearing methods provides a great potential for imaging entire central nervous system by combining with multiple-labelling and microscopic imaging techniques. These methods had made certain clearing contributions with respective weaknesses, including tissue deformation, fluorescence quenching, execution complexity and antibody penetration limitation that makes immunostaining of tissue blocks difficult. The passive clarity technique (PACT) bypasses those problems and clears the samples with simple implementation, excellent transparency with fine fluorescence retention, but the passive tissue clearing method needs too long time. In this study, we not only accelerate the clearing speed of brain blocks but also preserve GFP fluorescence well by screening an optimal clearing temperature. The selection of proper temperature will make PACT more applicable, which evidently broaden the application range of this method.

Paper Details

Date Published: 10 March 2015
PDF: 6 pages
Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 930527 (10 March 2015); doi: 10.1117/12.2078736
Show Author Affiliations
Tingting Yu, Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
MoE Key Lab. for Biomedical Photonics (China)
Yisong Qi, Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
MoE Key Lab. for Biomedical Photonics (China)
Hui Gong, Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
MoE Key Lab. for Biomedical Photonics (China)
Qingming Luo, Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
MoE Key Lab. for Biomedical Photonics (China)
Dan Zhu, Britton Chance Ctr. for Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
MoE Key Lab. for Biomedical Photonics (China)


Published in SPIE Proceedings Vol. 9305:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Henry Hirschberg; Steen J. Madsen; E. Duco Jansen; Qingming Luo; Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

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