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Proceedings Paper

New insights and system designs for temporally focused multiphoton optogenetics
Author(s): Tom Mayblum; Adi Schejter; Hod Dana; Shy Shoham
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Paper Abstract

Temporal focusing (TF) multiphoton systems constitute a powerful solution for cellular resolution optogenetic stimulation and recording in three-dimensional, scattering tissue. Here, we address two fundamental aspects in the design of such systems: first, we examine the design of TF systems with specific optical sectioning by comparatively analyzing previously published results. Next, we develop a solution for obtaining TF in a flexible three-dimensional pattern of cellmatched focal spots. Our solution employs spatio-temporal focusing (SSTF) in a unique optical system design that can be integrated before essentially any multiphoton imaging or stimulation system.

Paper Details

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932928 (5 March 2015); doi: 10.1117/12.2078678
Show Author Affiliations
Tom Mayblum, Technion-Israel Institute of Technology (Israel)
Adi Schejter, Technion-Israel Institute of Technology (Israel)
Hod Dana, Howard Hughes Medical Institute (United States)
Shy Shoham, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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