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Proceedings Paper

Method for observing phase objects without halos and directional shadows
Author(s): Yoshimasa Suzuki; Kazuo Kajitani; Hisashi Ohde
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Paper Abstract

A new microscopy method for observing phase objects without halos and directional shadows is proposed. The key optical element is an annular aperture at the front focal plane of a condenser with a larger diameter than those used in standard phase contrast microscopy. The light flux passing through the annular aperture is changed by the specimen's surface profile and then passes through an objective and contributes to image formation. This paper presents essential conditions for realizing the method.

In this paper, images of colonies formed by induced pluripotent stem (iPS) cells using this method are compared with the conventional phase contrast method and the bright-field method when the NA of the illumination is small to identify differences among these techniques. The outlines of the iPS cells are clearly visible with this method, whereas they are not clearly visible due to halos when using the phase contrast method or due to weak contrast when using the bright-field method. Other images using this method are also presented to demonstrate a capacity of this method: a mouse ovum and superimposition of several different images of mouse iPS cells.

Paper Details

Date Published: 11 March 2015
PDF: 7 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 93362G (11 March 2015); doi: 10.1117/12.2078571
Show Author Affiliations
Yoshimasa Suzuki, Olympus Corp. (Japan)
Kazuo Kajitani, Olympus Corp. (Japan)
Hisashi Ohde, Olympus Corp. (Japan)

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)

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