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Proceedings Paper

Computational modeling of STED microscopy through multiple biological cells under one- and two-photon excitation
Author(s): Andrew E. Mark; Mitchell A. Davis; Matthew S. Starosta; Andrew K. Dunn
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Paper Abstract

While superresolution optical microscopy techniques afford enhanced resolution for biological applications, they have largely been used to study structures in isolated cells. We use the FDTD method to simulate the propagation of focused beams for STED microscopy through multiple biological cells. We model depletion beams that provide 2D and 3D confinement of the fluorescence spot and assess the effective PSF of the system as a function of focal depth. We compare the relative size of the STED effective PSF under one- and two-photon excitation. PSF calculations suggest that imaging is possible up to the maximum simulation depth if the fluorescence emission remains detectable.

Paper Details

Date Published: 9 March 2015
PDF: 9 pages
Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93300V (9 March 2015); doi: 10.1117/12.2078345
Show Author Affiliations
Andrew E. Mark, The Univ. of Texas at Austin (United States)
Mitchell A. Davis, The Univ. of Texas at Austin (United States)
Matthew S. Starosta, Sandia National Labs. (United States)
Andrew K. Dunn, The Univ. of Texas at Austin (United States)


Published in SPIE Proceedings Vol. 9330:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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