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Proceedings Paper

Biological super-resolution imaging by using novel microsphere-embedded coverslips
Author(s): Arash Darafsheh; Consuelo Guardiola ; Deepak Nihalani; Daeyeon Lee; Jarod C. Finlay; Alejandro Cárabe
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Paper Abstract

Optical super-resolution imaging is a desirable technique in many fields, including medical and material sciences and nanophotonics. We demonstrated feasibility of optical microscopy, with resolution improvement by a factor of 2-3, by using microsphere-embedded coverslips composed of barium titanate glass microspheres (refractive index n~1.9-2.1) fixed in a transparent layer of polydimethylsiloxane. Imaging was performed by a conventional microscope and a fluorescent microscope with the microsphere-embedded layer placed in a contact position with various biological specimens and semiconductor nanostructures. We investigated a biomedical application of microsphere-assisted imaging technique by immunostaining of kidney sections where cellular distribution of a motor protein Myo1c and a podocyte specific protein ZO-1 was analyzed. A significant visual enhancement in the distribution pattern of the proteins was noted in the stained glomeruli by using microsphere-assisted imaging technique. Our results suggest that microsphere-assisted imaging technique is a promising candidate for applications in medical and cancer research, as well as in microfluidics and nanophotonics.

Paper Details

Date Published: 11 March 2015
PDF: 7 pages
Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 933705 (11 March 2015); doi: 10.1117/12.2078019
Show Author Affiliations
Arash Darafsheh, Univ. of Pennsylvania (United States)
Consuelo Guardiola , Univ. of Pennsylvania (United States)
Deepak Nihalani, Univ. of Pennsylvania (United States)
Daeyeon Lee, Univ. of Pennsylvania (United States)
Jarod C. Finlay, Univ. of Pennsylvania (United States)
Alejandro Cárabe, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 9337:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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