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Proceedings Paper

Microscope-on-chip: combining lens-free microscopy with integrated photonics
Author(s): Richard Stahl; Dries Vercruysse; Tom Claes; Geert Vanmeerbeeck; Vignesh Mukund; Roelof Jansen; Jeonghwan Song; Luis Hoffman; Xavier Rottenberg; Andy Lambrechts; Liesbet Lagae
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Paper Abstract

Lens-free in-line Holographic Microscopy (LHM) is a promising imaging technique for many biomedical and industrial applications. The main advantage of the technique is the simplicity of the imaging hardware, requiring no lenses nor high-precision mechanical components. Nevertheless, the LHM systems achieve high imaging performance only in combination with a high-quality and complex illumination. Furthermore, to achieve truly high-throughput imaging capabilities, many applications require a complete on-chip integration. We demonstrate the strength, versatility and scalability of our integrated approach on two microscopes-on-chip instances that combine image sensor technologies with photonics (and micro-fluidics): a fully integrated Point-Source (PS) LHM module for in-flow cell inspection and Large Field-of-View (LFoV) microscope with on-chip photonic illumination for large-area imaging applications. The proposed PS-LHM module consists of a photonic illumination, a micro-fluidic channel and an imager, integrated in a total volume smaller than 0.5 mm3. A low-loss single-mode photonic waveguide is adapted to generate a high- NA illumination spot. Experimental results show strong focusing capabilities and sufficient overall coupling efficiency. Current PS-LHM prototype reaches imaging resolution below 600nm. Our LFoV-LHM system is extremely vertically compact as it consists of only one 1mm-thick illumination chip and one 3mm-thick imaging module. The illumination chip is based on fractal-layout phase-matched waveguides designed to generate multiple light sources that create a quasi-planar illumination wavefront over an area few square millimeter large. Current illumination prototype has active area of approximately 1.2×1.2mm2. Our LFoV-LHM prototype reaches imaging resolution of 870nm using image sensor with 1.12μm pixel pitch with maximum FoV of 16.47mm2.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281C (2 March 2015); doi: 10.1117/12.2077484
Show Author Affiliations
Richard Stahl, IMEC (Belgium)
Dries Vercruysse, IMEC (Belgium)
Tom Claes, IMEC (Belgium)
Geert Vanmeerbeeck, IMEC (Belgium)
Vignesh Mukund, IMEC (Belgium)
Roelof Jansen, IMEC (Belgium)
Jeonghwan Song, IMEC (Belgium)
Luis Hoffman, IMEC (Belgium)
Xavier Rottenberg, IMEC (Belgium)
Andy Lambrechts, IMEC (Belgium)
Liesbet Lagae, IMEC (Belgium)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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