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Proceedings Paper

Photo-imprint super-resolution photoacoustic microscopy
Author(s): Junjie Yao; Lidai Wang; Chiye Li; Chi Zhang; Lihong V. Wang
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Paper Abstract

Combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for super-resolution photoacoustic imaging of both fluorescent and non-fluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. By scanning the excitation beam, we performed three-dimensional sub-diffraction imaging of varied fluorescent and non-fluorescent species. A lateral resolution of 80 nm and an axial resolution of 370 nm have been demonstrated. This technique has the potential to enable label-free super-resolution imaging, and can be transferred to other optical imaging modalities or combined with other super-resolution methods.

Paper Details

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 932327 (11 March 2015); doi: 10.1117/12.2077350
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Lidai Wang, Washington Univ. in St. Louis (United States)
Chiye Li, Washington Univ. in St. Louis (United States)
Chi Zhang, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 9323:
Photons Plus Ultrasound: Imaging and Sensing 2015
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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