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Proceedings Paper

A novel method for image denoising of fluorescence molecular imaging based on fuzzy C-Means clustering
Author(s): Yu An; Jie Liu; Jinzuo Ye; Yamin Mao; Xin Yang; Shixin Jiang; Chongwei Chi; Jie Tian
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Paper Abstract

As an important molecular imaging modality, fluorescence molecular imaging (FMI) has the advantages of high sensitivity, low cost and ease of use. By labeling the regions of interest with fluorophore, FMI can noninvasively obtain the distribution of fluorophore in-vivo. However, due to the fact that the spectrum of fluorescence is in the section of the visible light range, there are mass of autofluorescence on the surface of the bio-tissues, which is a major disturbing factor in FMI. Meanwhile, the high-level of dark current for charge-coupled device (CCD) camera and other influencing factor can also produce a lot of background noise. In this paper, a novel method for image denoising of FMI based on fuzzy C-Means clustering (FCM) is proposed, because the fluorescent signal is the major component of the fluorescence images, and the intensity of autofluorescence and other background signals is relatively lower than the fluorescence signal. First, the fluorescence image is smoothed by sliding-neighborhood operations to initially eliminate the noise. Then, the wavelet transform (WLT) is performed on the fluorescence images to obtain the major component of the fluorescent signals. After that, the FCM method is adopt to separate the major component and background of the fluorescence images. Finally, the proposed method was validated using the original data obtained by in vivo implanted fluorophore experiment, and the results show that our proposed method can effectively obtain the fluorescence signal while eliminate the background noise, which could increase the quality of fluorescence images.

Paper Details

Date Published: 9 March 2015
PDF: 6 pages
Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93301E (9 March 2015); doi: 10.1117/12.2077141
Show Author Affiliations
Yu An, Beijing Jiaotong Univ. (China)
Jie Liu, Beijing Jiaotong Univ. (China)
Jinzuo Ye, Key Lab. of Molecular Imaging (China)
Yamin Mao, Key Lab. of Molecular Imaging (China)
Xin Yang, Key Lab. of Molecular Imaging (China)
Shixin Jiang, Beijing Jiaotong Univ. (China)
Chongwei Chi, Key Lab. of Molecular Imaging (China)
Jie Tian, Key Lab. of Molecular Imaging (China)


Published in SPIE Proceedings Vol. 9330:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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