Share Email Print
cover

Proceedings Paper

Four-wave mixing microscopy: a high potential nonlinear imaging method
Author(s): Tobias Ehmke; Andreas Knebl; Alexander Heisterkamp
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.

Paper Details

Date Published: 5 March 2015
PDF: 10 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932912 (5 March 2015); doi: 10.1117/12.2076743
Show Author Affiliations
Tobias Ehmke, Laser Zentrum Hannover e.V. (Germany)
Andreas Knebl, Friedrich-Schiller-Univ. Jena (Germany)
Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany)
Leibniz Univ. Hannover (Germany)


Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

© SPIE. Terms of Use
Back to Top