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Proceedings Paper

Flavin fluorescence lifetime imaging of living peripheral blood mononuclear cells on micro and nano-structured surfaces
Author(s): Tibor Teplicky; J. Horilova; J. Bruncko; C. Gladine; I. Lajdova; A. Mateasik; D. Chorvat; A. Marcek Chorvatova
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Paper Abstract

Fabricated micro- and nano-structured surfaces were evaluated for use with living cells. Metabolic state was tested by means of endogenous flavin fluorescence of living peripheral blood mononuclear cells (PBMC) positioned on a coverslip, non-covered, or covered with micro- or nano-structured surfaces (OrmoComp polymer structures produced by 2-photon photopolymerisation, or Zinc Oxide (ZnO) layer fabricated by pulsed laser deposition). Confocal microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) were employed to gather flavin fluorescence lifetime images of living PBMC on structured surfaces. Gathered data are the first step towards monitoring of the live cell interaction with different micro/nano-structured surfaces and thus evaluate their potential applicability in the biomedical field.

Paper Details

Date Published: 5 March 2015
PDF: 10 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290D (5 March 2015); doi: 10.1117/12.2076706
Show Author Affiliations
Tibor Teplicky, International Laser Ctr. (Slovakia)
Univ. of Ss. Cyril and Methodius (Slovakia)
J. Horilova, International Laser Ctr. (Slovakia)
Pavol Jozef Safarik Univ. (Slovakia)
J. Bruncko, International Laser Ctr. (Slovakia)
C. Gladine, Clermont Univ., Univ. d'Auvergne, Unité de Nutrition Humaine (France)
I. Lajdova, Slovak Medical Univ. (Slovakia)
A. Mateasik, International Laser Ctr. (Slovakia)
D. Chorvat, International Laser Ctr. (Slovakia)
A. Marcek Chorvatova, International Laser Ctr. (Slovakia)
Univ. of Ss. Cyril and Methodius (Slovakia)


Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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